SDS-PAGE explained - Protein Separation Technique
4:05
YouTubeHenrik's Lab
SDS-PAGE explained - Protein Separation Technique
Hey Friends, SDS-PAGE (Sodium Dodecyl Sulfate PolyAcrylamide Gel Electrophoresis) is used to separate proteins in a sample according to their molecular weight. SDS ...
327.3K viewsFeb 1, 2021
SDS-PAGE Protocol
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Western Blot Protocol | Bio-Rad
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Separating Protein with SDS-PAGE: Procedure and Applications
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SOLVED: Calculate the preparation of all samples to be run on your SDS-PAGE gel. You need to load 10 μg total protein in 3 μl. You need to prepare 30 μl of this solution that has protein and 1X sample buffer (water is used for dilution). Complete the table below.Sample Protein Protein Water 5X Sample Total Sample Conc. Sample (μg) Buffer Volume (μl) (μl) (μl)CRUDE 30Desalt 30Peak Fraction 30Peak Fraction 30Note: You must complete this table before coming to class.
SOLVED: Calculate the preparation of all samples to be run on your SDS-PAGE gel. You need to load 10 μg total protein in 3 μl. You need to prepare 30 μl of this solution that has protein and 1X sample buffer (water is used for dilution). Complete the table below.Sample Protein Protein Water 5X Sample Total Sample Conc. Sample (μg) Buffer Volume (μl) (μl) (μl)CRUDE 30Desalt 30Peak Fraction 30Peak Fraction 30Note: You must complete this table before coming to class.
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